The new financial sanctions target 21 entities including subsidiaries of the state-owned atomic energy corporation Rosatom, which took over a Ukrainian nuclear power plant.
Three individuals have been targeted including the head of the federal financial monitoring service and two Rosatom board members who double as aides and advisers for President Vladimir Putin.
An export ban has been placed on all machinery and related parts to Russia and areas under Russian control to prevent Australian goods from aiding its invasion of Ukraine.
Upon arriving in Japan for the G7, Prime Minister Anthony Albanese said Australia stood by Ukraine's unwavering resilience and courage and again called on Russia to end the war.
He said Australia would continue to provide support to Ukraine, adding "the struggle of the Ukrainian people is a struggle for the international rule of law".
"The people of Ukraine are making enormous sacrifices in order to preserve their nation-state, their democracy and it's important that the world stand with Ukraine," he said.
"Will continue to liaise with them about what further support we can give."
He said discussions with G7 nations and partners would also address the wider impacts of Russia's invasion, including food and energy security.
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